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Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different <t>colorectal</t> cancer cell lines <t>(HCT116,</t> HT‐29, <t>Caco2,</t> Widr, and <t>SW480)</t> after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
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caco-2 human colorectal cancer cell line  (Korean Cell Line Bank)
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Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different <t>colorectal</t> cancer cell lines <t>(HCT116,</t> HT‐29, <t>Caco2,</t> Widr, and <t>SW480)</t> after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
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Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different <t>colorectal</t> cancer cell lines <t>(HCT116,</t> HT‐29, <t>Caco2,</t> Widr, and <t>SW480)</t> after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
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human colorectal cancer cell line caco-2  (Korean Cell Line Bank)
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Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different <t>colorectal</t> cancer cell lines <t>(HCT116,</t> HT‐29, <t>Caco2,</t> Widr, and <t>SW480)</t> after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.
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Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different colorectal cancer cell lines (HCT116, HT‐29, Caco2, Widr, and SW480) after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Journal: Cancer Science

Article Title: KLHL5 Contributes to Colorectal Cancer Cell Survival by Promoting Cell Cycle Progression and Suppressing Apoptotic Cell Death

doi: 10.1111/cas.70108

Figure Lengend Snippet: Effect of KLHL5 knockdown on mRNA expression, cell viability, migration, and anoikis in CRC cell lines. Relative expression of KLHL5 mRNA in different colorectal cancer cell lines (HCT116, HT‐29, Caco2, Widr, and SW480) after KLHL5 knockdown using siRNA. KLHL5 mRNA knockdown using specific siRNA in HCT116 and SW480 cells resulted in reduced KLHL5 mRNA expression in both cell lines. Effect of KLHL5 knockdown on the viability of HCT116 and SW480 cells using the MMT assay. Effect of KLHL5 knockdown on HCT116 and SW480 cell numbers using the anoikis assay. The impact of KLHL5 knockdown on cell migration in HCT116 and SW480 cells. Statistical significance: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: Human colorectal cancer cell lines (HCT116, HT‐29, SW480, Caco2, and WiDr) were provided by Nagoya City University (American Type Culture Collection (ATCC), Manassas, VA, USA).

Techniques: Knockdown, Expressing, Migration